佛山科学技术学院学报：自然科学版2011，Vol.29Issue(4)：47-51,5.
流式细胞术检测不同N/P条件下藻细胞DNA含量
DNA content detection of microcystis aeruginosa PCC 7806 on different N/P nutrients by FCM
陈江莲 1章军 2徐虹1
作者信息
- 1. 韶关学院,广东韶关512005
- 2. 厦门大学生命科学学院,福建厦门361005
- 折叠
摘要
Abstract
In the formation of algae bloom,the proportion of nitrogen and phosphorus is the major influential factor.The optimal N/P for alage is 16：1.It is assumed that the particular proportion be related to the growth and division of cyanobacteria cells.The most vital role in vivo of phosphorus is involved in DNA、RNA synthesis.As for nitrogen,it is related to the protein synthesis.The appropriate ratio between N and P may be presumed as a reflection of the relation in quantity between the growth and division of alage cell.In order to verify the assumption,we used the technology of FCM to detect the cells of M.aeruginosa stained by PI which were cultured on different N/P nutrients and set up four groups of different N/P ratios,16∶1,160∶1,1∶1 and lacking of NP.It was found that when N/P=16∶1,the DNA content of M.aeruginosa cells is higher than others.We concluded that the division of these cells is at an optimal level.关键词
流式细胞术/N/P/DNA含量测定Key words
flow cytometry（FCM）/N/P/DNA content detection分类
生物工程引用本文复制引用
陈江莲,章军,徐虹..流式细胞术检测不同N/P条件下藻细胞DNA含量[J].佛山科学技术学院学报：自然科学版,2011,29(4):47-51,5.
