佛山科学技术学院学报：自然科学版2011，Vol.29Issue(5)：82-85,4.
烛缸法分离培养及冷冻法保存幽门螺杆菌
Candle jar fractional cultivation and cryopreservation of Helicobacter pylori
摘要
Abstract
Objects To study the simple method for the separation and reservation of Helicobacter pylori（H pylori） and construct a small storage of H pylori.Method Firstly,the candle jar method was used to separate samples of H pylori from different sources by H pylori selective medium,Secondarily,the Gram microscopy test and biochemical experiment were used to identify H pylori.Thirdly,the pure H pylori was reserved in three different preservation solutions（brucella broth,50% glycerol serum,50% glycerol egg white） which were 20 °C below zero.Finally,the samples were tested to find out the survival rate after 1-,6-,12-day reservation.Result Among the 21 H pylori samples,of which 13 were collected and obtained from 58 clinical specimens and the other 8 were from oral and fecal samples of 56 undergraduates,the survival rate of 21 of the samples was over 90%.That is,the survival rate showed no significant difference when they were tested after 1-and 6-day reservation.The survival rate of the samples reserved in different solutions showed significant different when tested in 12-day reservation： that of those in 50% glycerol serum was over 80%,that of those in the other solutions was less than 50%.Conclusion Candle jar method can be used as a good way to isolated H pylori and the 50% glycerol which is 20 °C below zero can reserve H pylori well for more than 12 days.It is convenient to construct a simple and small storage of H pylori and valuable in scientific research by using the bacteria from the storage.关键词
幽门螺杆菌/分离培养/菌种/菌种收藏Key words
Helicobacter pylori/fractional cultivation/bacterial isolates/type culture collection分类
生物科学引用本文复制引用
万学勤,周鹏志,马峰,李万可兰,谭淑仪,邝佳..烛缸法分离培养及冷冻法保存幽门螺杆菌[J].佛山科学技术学院学报：自然科学版,2011,29(5):82-85,4.基金项目
广东省自然科学基金项目 （）
广东省科技厅社会发展项目 （）
