分析测试学报2011，Vol.30Issue(12)：1345-1350,6.DOI:10.3969/j.issn.1004-4957.2011.12.003
超顺磁性免疫磁珠体系用于植物油中黄曲霉毒素B1的检测研究
Research of the Super Paramagnetic Immune Magnetic Beads System for Detection of Aflatoxin B1 in Vegetable Oil
摘要
Abstract
Using the method of chemical co-precipitation, the super paramagnetic nanoparticles with the key component of Fe3O4 were synthesized. The magnetic beads reacted with the silane coupling a-gent 3-aminopropyl triethoxysilane and connected with amino groups. After activated by the agent gl-utaraldehyde, the magnetic beads took along the aldehyde group and coupled with aflatoxin B, poly-clonal antibody to form the immune magnetic beads. A method was established to detect the aflatoxin B, in vegetable oil by using the organic solvent for preliminary extraction, the aflatoxin B, immune magnetic beads system to purification and HPLC to quantification. The method showed a good linearity in the range of 5 - 50 p:g/L with correlation coefficient of 0. 999 4 and detection limit of 0. 5 |xg/L. The average recovery was up to 96% and the RSD was 12. 5% . With the advantages of simplicity, sensitivity and high accuracy, the method could be applied in the determination of aflatoxin B1 in vegetable oil.关键词
免疫磁珠/植物油/黄曲霉毒素B1Key words
immune magnetic beads/ vegetable oil/ aflatoxin B,分类
化学化工引用本文复制引用
刘伟伟,孙秀兰,张银志,樊惠良,陈文君,李在均..超顺磁性免疫磁珠体系用于植物油中黄曲霉毒素B1的检测研究[J].分析测试学报,2011,30(12):1345-1350,6.基金项目
国家自然科学基金资助项目(20806033) （20806033）
张家港市科技局资助项目(ZKS0904) （ZKS0904）
