分析测试学报2011，Vol.30Issue(12)：1387-1391,5.DOI:10.3969/j.issn.1004-4957.2011.12.011
高效液相色谱同时测定3类食品中的5种罂粟壳生物碱
Determination of 5 Opium Poppy Husk Alkaloids in Three Types of Food by HPLC
摘要
Abstract
A high performance liquid chromatographic ( HPLC ) method was developed for the determination of morphine, codeine, thebaine, papaverine and narcotine in three types of food including spices class, halogen soups and cooked food categories. The food samples were ultrasonically extracted with ethanol, and then separated on a C18 column(250 mm x 4. 6 mm, 0. 5 jxm) with methanol -0.01 mol ? L-1 phosphate buffer as mobile phase at a flow rate of 1.0 mL ? Min-1. The UV wavelength was set at 250 nm. The result indicated that the standard curves of five alkaloids were linear in the range of 10 -600 mg ? L-1 with correlation coefficients not less than 0. 999 6. The detection limits of five alkaloids were between 0. 1 mg ? Kg and 0. 6 mg ? Kg . The average recoveries of 5 alkaloids from three matrices at high and low spiked levels ranged from 88% to 108% , with relative standard deviations( RSD, n =6) ranged from 0. 77% to 10. 7% . The method was simple, sensitive, accurate and repeatable, and could be used for the determination of five alkaloids in food sample.关键词
罂粟壳/生物碱/高效液相色谱/食品Key words
poppy/ alkaloid/ HPLC / food分类
化学化工引用本文复制引用
徐丽红,王建清,陶秋,张玉,王伟..高效液相色谱同时测定3类食品中的5种罂粟壳生物碱[J].分析测试学报,2011,30(12):1387-1391,5.基金项目
浙江省科技厅重大科技专项项目(2009C12052) （2009C12052）
浙江省农业科学院重点实验室前瞻性项目(2009R19Y01D01) （2009R19Y01D01）
