分析测试学报2011，Vol.30Issue(12)：1400-1405,6.DOI:10.3969/j.issn.1004-4957.2011.12.014
2-羟基苯乙酮铜(Ⅱ)配合物与ct-DNA的键合作用研究
Study on Binding Interaction between ct-DNA and Copper( Ⅱ ) Complex of 2-Hydroxy Acetophenone
摘要
Abstract
In this study, the binding mode of a synthesized copper( II ) complex of 2-hydroxy aceto-phenone(HAP) , [ Cu(HAP)2 ] (1) , to calf thymus DNA(ct-DNA) as biomolecular target was discussed by UV - Vis absorption, fluorescence emission and circular dichroism spectra, as well as DNA viscosity titration experiment. Results indicated that complex 1 binds to ct-DNA mainly via intercalation and exterior electrostatic interaction, in which intercalation can be ascribed to aromatic ring of HAP and the rigid planar structure of whole complex 1, while electrostatic interaction to DNA polyanionic backbone should be attributed to the cationic copper ( H ) center of complex 1. The research results provided scientific evidence for the further exploration of the pharmaceutical activities of serial metal complexes of HAP.关键词
2-羟基苯乙酮/铜(Ⅱ)配合物/小牛胸腺DNA/光谱分析/插入作用Key words
2-hydroxy acetophenone/ copper ( E ) complex/ ct-DNA/ spectral analysis/ intercalation分类
化学化工引用本文复制引用
刘延成,王海璐,梁炜乾,农秀连,陈振锋..2-羟基苯乙酮铜(Ⅱ)配合物与ct-DNA的键合作用研究[J].分析测试学报,2011,30(12):1400-1405,6.基金项目
国家自然科学基金项目(20861002) （20861002）
广西自然科学基金项目(0991012Z,0991003) （0991012Z,0991003）
广西师范大学重点项目(2010ZD007) （2010ZD007）
