分析测试学报2011，Vol.30Issue(12)：1425-1429,5.DOI:10.3969/j.issn.1004-4957.2011.12.018
高效液相色谱测定石榴皮水提取物中4种多酚化合物的含量
Determination of Four Polyphenolic Compounds in Pomegranate Peel Water Extracts by High Performance Liquid Chromatography
摘要
Abstract
To quantitative analyzing four polyphenolic compounds of gallic acid, punicalagin A, pu-nicalalgin B and ellagic acid in pomegranate peel water extracts, a high performance liquid chromato-graphic ( HPLC) method was established. The samples were prepared and filtered prior to the HPLC analysis. Quantification analysis of these analytes were achieved under the optimized HPLC conditions using the external standard method. The results showed that the calibration curves for gallic acid, punicalagin A, punicalagin B and ellagic acid were linear in the concentration ranges of 7. 48 -149.50, 15.36-153.55, 27.65-276.45, 7. 13 - 114. 00 mg/L, respectively, with their correlation coefficients(r2 ) greater than 0.99. The limits of detection( LOD) were 0. 12, 1.92, 3.46, 0. 22 mg/L, and the limits of quantitation( LOQ) were 0. 48, 7. 68 , 13. 83 , 0. 89 mg/L, respectively. The spiked recoveries ranged from 83% to 112% with relative standard deviations ( RSDs) less than 10% . The developed method was simple, sensitive, reliable and reproducible, and could be applied in the rapid determination of gallic acid, punicalagin A, punicalagin B and ellagic acid in pomegranate peel water extracts in 17 min.关键词
安石榴甙A和B/没食子酸/鞣花酸/石榴皮水提取物/高效液相色谱Key words
punicalagins A & B/ gallic acid/ ellagic acid/ pomegranate peel water extracts/ high performance liquid chromatography分类
化学化工引用本文复制引用
曲文娟,张渭洁,徐敏瑶,杨洋,蒋洁玮..高效液相色谱测定石榴皮水提取物中4种多酚化合物的含量[J].分析测试学报,2011,30(12):1425-1429,5.基金项目
江苏大学高级技术人才科研启动基金项目(10JDG121) （10JDG121）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
江苏大学学生科研立项项目(10A223) （10A223）
江苏大学博士后基金项目(1143002027) （1143002027）
江苏省博士后科研基金项目(1631360008) （1631360008）
