分析测试学报2012，Vol.31Issue(1)：45-50,6.DOI:10.3969/j.issn.1004-4957.2012.01.008
高速逆流色谱法分离紫锥菊花色苷及其抗氧化性研究
Separation of Anthocyanins in Echinacea Purpurea Flower by High Speed Counter-current Chromatography and the Research on the Antioxidant Activity of the Anthocyanins
摘要
Abstract
An efficient method for the isolation and preparation of two anthocyanins, cyaniding 3-0-(β-D-giucopyranoside) (compound 1) and cyaniding 3-0-(6"-0-malonyl-β-D-glucopyranoside) (compound 2) , from Echinacea purpurea flower by high speed counter-current chromatography ( HSCCC ) was established. The fresh flower was extracted with 60% ethanol (containing 0. 1% HC1) at low temperature and dark conditions. 2. 1 g of crude sample for HSCCC was prepared after extracted with ethyl acetate and passed through an adsorbing column (2 cm x 30 cm, containing 100 mL D101 macroporous resin). Then water - n-butanol - methyl tert-butyl ether - acetonitrile - trifluoroacetic acid(6 : 3 : 2 : 1 : 0. 001) was used as the two-phase solvent system, in which the upper phase was used as the stationary phase, while the lower phase was used as the mobile phase, the flow rate was set at 2. 0 mL/min, and the revolution speed was 850 r/min. 9. 8 mg compound 1 with purity of 95. 1% and 14. 3 mg compound 2 with purity of 98. 2% were obtained from 160 mg crude sample by one-step, as determined by high-performance liquid chromatography ( HPLC ). The chemical structures were identified by mass spectrometry ( MS ) and -nuclear magnetic resonance imaging ( NMR). Additionally, the antioxidant activities of the two compounds were also evaluated by the method of 1 ,l-diphenyl-2-picrylhydrazyl( DPPH μ) radical scavenging assay with Vc as the control group. The results of antioxidative activity test showed that the two compounds have strong capacity of scavenging DPPH(EC50 < 10 mg/L). Both of their EC50 were lower than that of Vc, and the scavenging ability of compound 1 was stronger than that of compound 2.关键词
高速逆流色谱/紫锥菊/花色苷/DPPH·/抗氧化性Key words
high speed counter-current chromatography/Echinacea purpurea/anthocyanins/DPPH ? : antioxidant activity分类
化学化工引用本文复制引用
李佳银,罗晋,李觅路,陆英,刘仲华..高速逆流色谱法分离紫锥菊花色苷及其抗氧化性研究[J].分析测试学报,2012,31(1):45-50,6.基金项目
湖南省重大科技专项资助(NK07006) （NK07006）
