分析测试学报2012，Vol.31Issue(1)：56-61,6.DOI:10.3969/j.issn.1004-4957.2012.01.010
卷烟主流烟气中8种羰基化合物的超高效液相色谱测定
Determination of Major Carbonyls in Mainstream Cigarette Smoke by Ultra Performance Liquid Chromatography
摘要
Abstract
An Ultra Performance Liquid Chromatographic (UPLC ) method was developed for the separation and determination of formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone, butyraldehyde in cigarette mainstream smoke. The targeted compounds in cigarette mainstream smoke were collected with a Cambridge fliter treated with acidic solution of 2, 4-dinitrophenylhydrazine, and extracted with 50 Ml 2% pyridine acetonitrile. The carbonyl compounds were separated on a KinetexTM C18 (150 mm ×2. 1 mm, 2. 6 μm) column, using water - acetonitrile (35 : 65) mixed solution and water - acetonitrile - tetrahydrofuran - isopropyl alcohol (59 : 30 : 10 : 1) as mobile phases by gradient elution. The eight carbonyl compounds were separated in 20 min and determined by UPLC with a diode array detector. The result indicated that the correlation coefficients (r ) of eight carbonyl compounds were not less than 0. 999 97. The detection limits for the method were between 25. 81 ng/cig and 67. 74 ng/cig. The average recoveries were in the range of 95% -99% with relative standard deviations of 1. 4% -5. 8% . Compared with the YC/T 254 -2008 standard method, the proposed method was faster, simpler and less consumption, and was applied in the determination of eight carbonyl compounds in twenty kinds of cigarette with satisfactory results.关键词
卷烟/主流烟气/超高效液相色谱/羰基化合物Key words
cigarette/mainstream smoke/ultra performance liquid chromatography ( UPLC) /carbonyl compounds分类
化学化工引用本文复制引用
李响丽,李国智,范多青,杨仁礼,毛多斌..卷烟主流烟气中8种羰基化合物的超高效液相色谱测定[J].分析测试学报,2012,31(1):56-61,6.基金项目
云南中烟科技项目资助(2011JC07) （2011JC07）
