分析测试学报2012，Vol.31Issue(1)：71-75,5.DOI:10.3969/j.issn.1004-4957.2012.01.013
4种黄酮小分子对DPPH自由基的清除作用及构效关系研究
Structure-Activity Relationship of Four Flavonoids on Scavenging Activities to DPPH Free Radical
摘要
Abstract
The scavenging activities, stabilities and hemi-inhibitory concentrations ( IC50 ) of 4 fla-vonoids, including rutin, vitexin, kaempferide and hyperoside to DPPH free radical were investigated by UV - Vis spectra, by using natural antioxidants ascorbic acid as the comparison. The relations of the flavonoids structures with their antioxidant activities were also discussed. The results indicated that different antioxidants have different balance time in scavenging the DPPH free radical, in which rutin need the longest time. Four flavonoids and ascorbic acid have scavenging effect to DPPH free radical, and the dose-effect relationship exists. The DPPH free radical scavenging abilities of these five antioxidants were as follows; hyperoside > ascorbic acid > rutin > kaempferide > vitexin. The a-nalysis on the structures of four flavonoids showed that the pyrocatechol hydroxy group of B ring was necessary for the antioxidant activities of flavonoids compounds. The hydroxymethylation of - OH at B ring and the glycosylation of - OH at A ring were not favorable for antioxidant activities. The gly-cosylation of 3-OH at C ring could increase the antioxidant activities of flavonoids, in which the antioxidant activity of monloglycoside ( e. G. Hyperoside) is better than that of diglycoside (e. G. Rutin).关键词
黄酮小分子/DPPH自由基/构效关系Key words
flavonoids/ DPPH free radical/ structure - activity relationship分类
化学化工引用本文复制引用
刘帅涛,陶慧林,李锦艳..4种黄酮小分子对DPPH自由基的清除作用及构效关系研究[J].分析测试学报,2012,31(1):71-75,5.基金项目
广西教育厅科研资助项目(200708 LX339) （200708 LX339）
