分析测试学报2012，Vol.31Issue(1)：96-99,4.DOI:10.3969/j.issn.1004-4957.2012.01.018
铽-依诺沙星-SDS敏化荧光猝灭法检测雷公藤红素
Determination of Celastrol by Lanthanide Sensitized Fluorescence Quenching on Terbium-Enoxacin-SDS
摘要
Abstract
In NH3 - NH4C1 buffer solution (pH 9. 3 ) , terbium ( M ) reacted with enoxacin (ENX) to form fluorescence coordinate compound with the maximum excitation wavelength of 330 nm and the maximum emission wavelength of 545 nm. The presence of SDS obviously strengthened the fluorescence intensity, but the fluorescence intensity of the complex was diminished by continuously adding celastrol, while the locations of the excitation and emission wavelengths were kept the same. Based on this, a new fluorescence method was proposed for the determination of celastrol. The method is simple, rapid and sensitive. The linear range of celastrol is 5. 2 x 10-6 - 8. 4 x 10 -5 mol/L and the detection limit is 4. 1 x 10 -8 mol/L.关键词
雷公藤红素/铽/依诺沙星/SDS/荧光猝灭Key words
celastrol/ terbium/ enoxacin/ SDS/ fluorescence quenching分类
化学化工引用本文复制引用
吕菊波..铽-依诺沙星-SDS敏化荧光猝灭法检测雷公藤红素[J].分析测试学报,2012,31(1):96-99,4.基金项目
山东省高等学校科技计划项目(J10LF02,J11LB03) （J10LF02,J11LB03）
国家自然科学基金项目(21104030) （21104030）
