分析测试学报2012，Vol.31Issue(2)：121-126,6.DOI:10.3969/j.issn.1004-4957.2012.02.001
一种核糖核酸酶构象稳定性的研究
Study on the Conformational Stability of a Ribonuclease
摘要
Abstract
Aspergillus niger SA-13-20 is a new ribonuclease( Rnase) isolated from the extracellular enzymes secreted by a mutant named A. Niger SA-13-20. The conformational stability of A. Niger SA-13-20 ribonuclease at different pH was studied by ultraviolet (UV) , fluorescence and Fourier transform infrared (FT - IR) spectroscopic techniques. UV and fluorescence spectra showed that the conformation of this protein was stable at acid and weak alkali pH, but unstable at pH above 9. 6. The secondary structure of the Rnase was determined based on the analysis of amide I band of FT - IR spectra by using deconvolution and curve-fitting technology. The results showed that the percentages of a-helix, 0-sheet, turn angle and unordered structure were 13.28%, 42.30%, 26.48% and 17.95% at pH 5.0 at room temperature, respectively. Melting temperature (Tm), melting entropy (AS.) and melting enthalpy( AHJ of this Rnase were 70.1 °C, 644 J · Mol-1 K"1 and 22.1 kj Mol-1, respectively, which indicated that the Rnase belonged to a ribonuclease with higher heat tolerance. These results are helpful to understand the relationship between the structure and the function of this enzyme, and propel its application in the scientific research and industrial production.关键词
核糖核酸酶/构象稳定性/紫外光谱/荧光光谱/红外光谱Key words
ribonuclease/ conformational stability/ UV/ fluorescence spectrum/ FT - IR分类
化学化工引用本文复制引用
熊亚红,邓乃康,简晓亮,陈祖强,周建平..一种核糖核酸酶构象稳定性的研究[J].分析测试学报,2012,31(2):121-126,6.基金项目
华南农业大学"211工程"重点学科三期建设项目(2009B010100001) （2009B010100001）
国家自然科学基金项目(20906034B0604) （20906034B0604）
国家林业局计划项目(2011-4-75) （2011-4-75）
