分析化学2011，Vol.39Issue(11)：1634-1640,7.DOI:10.3724/SP.J.1096.2011.01634
基于一维DNA组装磁性纳米探针的夹心型安培免疫传感器研究
A Sandwich Amperometric Immunosensor Based on One-dimensional Assembly of Magnetic DNA Nano-probe
摘要
Abstract
A new type of one-dimensional magnetic nanoprobes was fabricated based on a DNA chain fixed with a number of Fe3O4 (core)/ZrO2 (shell) nano-magnetic beads (ZMPs) labeled with secondary antibody. The a-fetoprotein (AFP) was analyzed by using probes DNA/(ZMPs-HRP-AFP Ab2)n and immobilizing a-fetoprotein antibody (AFP Ab,) onto the glassy carbon electrode modified by nano-gold. The sandwich-type immunocomplex could be formed between the immobilized AFP Abi on the GCE and the probes DNA/( ZMPs-HRP-AFP Ab2) n and the carried HRP could catalyze the electrochemical reduction of carbamide peroxide (CP) with the help of hydroquinone (HQ). The novel one-dimensional nano structure assemble and sandwich-type amperometric immunosensor was developed. Enhanced sensitivity was achieved by introducing more HRP-AFP Ab2 onto the electrode surface through the pearl chain structure. Under optimal conditions, the proposed method could respond to 4 ng/L AFP with a linear calibration range from 0.01 to 25 ug/L. The immunosensor was employed to determine AFP in serum samples and the results were satisfactory. The proposed amperometric immunosensor was sensitive, quick, magnetic field controllable. This study provided a platform technology for developing a one-dimensional assembly and highly sensitive immunosensor, which was suitable for screen determination of tumors in serums of normal patients.关键词
脱氧核糖核酸/Fe3 O4/ZrO2/一维磁性纳米探针/甲胎蛋白/夹心型安培免疫传感器Key words
Deoxyribonucleic acid/ Ferroferric oxide/zirconium dioxide/ One dimensional magnetic nanoprobes/ crFetoprotein/ Sandwich electrochemical immunosensor引用本文复制引用
巫远招,干宁,胡富陶,李天华,曹玉廷,郑磊..基于一维DNA组装磁性纳米探针的夹心型安培免疫传感器研究[J].分析化学,2011,39(11):1634-1640,7.基金项目
本文系国家自然科学青年基金(No.20805024),广东省科技计划项目(Nos.2010A0303000006,2008A050200006)和宁波大学王宽诚基金资助项目 （No.20805024）
