分析化学2011，Vol.39Issue(11)：1711-1715,5.DOI:10.3724/SP.J.1096.2011.01711
基于双分子识别荧光猝灭法高选择性测定多巴胺
Fluorescence Quenching Method for Determination of Dopamine Based on Double Molecular Recognition
摘要
Abstract
CdTe quantum dots probe with both 4-mercaptophenylboronic acid and dithiobis(succinimi-dylpropionate) was designed and functionalized, and characterized by XPS and TEM. Emission spectra of CdTe quantum dots probe and dopamine was scanned under 431 nm excitation wavelength. The maximum emission wavelength shift red. At the maximum emission wavelength, dopamine results in fluorescence quenching of the probe. A direct, selective, and sensitive strategy for the determination of dopamine was established. The effects of determination conditions were investigated. Under the optimal conditions, a good linearity (R2 =0. 987) was obtained between fluorescence intensity and dopamine concentration in the range of 0. 02 - 20. 0 μmol/L. The linear regression equation was △F= 20. L + 31.7C(μmol/L). The limit of detection was 0. 0049 μmol/L. The method has been successfully applied to determine dopamine in injection, serous and urine with satisfactory result.关键词
双分子识别/荧光猝灭法/CdTe/量子点/多巴胺Key words
Double molecular recognition/ Fluorescence quenching method/ Cadmium telluride/ Quantum dots/ Dopamine引用本文复制引用
颜梅,葛慎光,卢娟娟,于京华..基于双分子识别荧光猝灭法高选择性测定多巴胺[J].分析化学,2011,39(11):1711-1715,5.基金项目
本文系国家自然科学基金(Nos.21175058,51003039),山东省自然科学基金(No.ZR2011BQ019),山东省科技发展计划(Nos.2011GGB01153,2009GG20003022)资助 （Nos.21175058,51003039）
