分析化学2011，Vol.39Issue(11)：1758-1761,4.DOI:10.3724/SP.J.1096.2011.01758
毛细管区带电泳测定D1蛋白酶的活性及其抑制剂先导化合物的筛选
Determination of D1 Protease Activity and Screening for Lead Compounds of Inhibitor by Capillary Zone Electrophoresis
摘要
Abstract
Capillary zone electrophoresis (CZE) with UV detection has been used for the determination of Dl protease activity and screening for lead compounds of inhibitor. Separation conditions of peptides were optimized as phosphate buffer (50 mmol/L, pH 3. 0) at 18 kV running voltage, with capillary length of 43 cm ×75 μm (id). Hydrolysis activity of Dl protease and screening for lead compounds of inhibitor was performed by CZE. The results indicated that two lead compounds ITP 21 and ITP 26 showed inhibitory effects of 26% and 13% , respectively, while other compounds had no inhibitory effects on Dl protease activity. This method is simple and convenient, suitable for the screening study on protease's inhibitors.关键词
D1蛋白酶/毛细管区带电泳/活性/先导化合物Key words
Protease/ Capillary zone electrophoresis/ Activity, Lead compounds引用本文复制引用
陈腊月,李伟国,黄雪英,刘中来,刘艳丽,冯璐,廖宏珊,熊国梅,祁超..毛细管区带电泳测定D1蛋白酶的活性及其抑制剂先导化合物的筛选[J].分析化学,2011,39(11):1758-1761,4.基金项目
本文系国家自然科学基金(Nos.20702017,30670429),湖北省自然科学基金(Nos.2007ABA141,2010CDB01206,2009CDB234)资助 （Nos.20702017,30670429）
