分析化学2011，Vol.39Issue(12)：1798-1804,7.DOI:10.3724/SP.J.1096.2011.01798
液相色谱-电喷雾-四级杆-飞行时间质谱法分析琼胶寡糖
Analysis of Agaro-oligosaccharide by Liquid Chromatography Coupled with Electrosprav Ionization-Quadrupole-Time of Flight-Mass Spectrometry
摘要
Abstract
A method was developed to isolate and identify agaro-oligosaccharides by ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS). In the experiment, three chromatographic columns (BEH Amide, BEH C8 and Atlantis T3) were used to isolate agaro-oligosacchrides. It was found that the BEH Amide had excel-lent isolation effect. Agarobiose oligomers with degree of polymerization (DP) ranging from 3 to 29 could be isolated rapidly and sensitively, and no derivatization procedure was needed. However, after derived with α-naphthylamine, agarobiose oligomers could be separated better by Atlantis T3 column, and a perfect AU spectrum was obtained. When combined liquid chromatography with ESI-Q-TOF-MS, accurate structure information of each chromatogram peak was obtained, the spectra were clear and the molecular weight of those oligosacchandes could be assigned easily.关键词
琼胶寡糖/BEH Amide色谱柱/液相色谱/电喷雾-四级杆-飞行时间质谱Key words
Agaro-oligosaccharides/ Amide chromatogram column/ Liquid chromatography/ Electros-pray ionization-quadrupole-time of flight-mass spectrometry引用本文复制引用
许艳婷,王秀娟,苏小玲,徐继林,陈海敏,陈娟娟,严小军..液相色谱-电喷雾-四级杆-飞行时间质谱法分析琼胶寡糖[J].分析化学,2011,39(12):1798-1804,7.基金项目
本文系教育部长江学者与创新团队项目(No.IRT0734)、浙江省公益性项目(No.2010C32021)、教育部博士点基金(No.200816460002)资助 （No.IRT0734）
