分析化学2011，Vol.39Issue(12)：1817-1822,6.DOI:10.3724/SP.J.1096.2011.01817
脱氧核糖核酸-蛋白质相互作用产物的检测及其在胃癌组织中的应用
Detection Method of Deoxyribonucleic Acid-Protein Interaction Products and Its Application in Gastric Cancer Tissue
摘要
Abstract
A method was established to separate DNA-protein interaction products by non-gel sieving capillary electrophoresis with laser induced fluorescence (NGS-CE-LIF). A series of effects on CE were studied, namely sieving medium concentration, separation temperature, separation voltage, pH value of Tris-Borate-EDTA (TBE) buffer. The results indicated that pUC19 DNA/MspⅠ(HpaⅡ) DNA marker and protein molecular weight marker (Broad) D532S interaction products were effectively separated when sieving medium was 0. 1% (ω/V) poly (ethylene oxide) ( 300000), electrophoresis buffer was 1×TBE (pH9. 0), separation voltage was 15 kV, and temperature was 15 ℃. The detection method of DNA-protein interaction products was used to analyze the interaction of the products of PCR of p53 gene and protein complex from gastric cancer tissue. The method has high detection rate and good separation efficiency.关键词
脱氧核糖核酸/蛋白质/相互作用/无胶筛分毛细管电泳/激光诱导荧光检测/聚环氧乙烷/胃癌组织Key words
Deoxyribonucleic acid/ Protein interaction/ Non-gel sieving capillary electrophoresis/ Laser-induced fluorescence detection/ Poly(ethylene oxide)/ Gastric cancer tissue引用本文复制引用
张爱梅,孙坤,王荣,谢华,谢希晖,施有琴..脱氧核糖核酸-蛋白质相互作用产物的检测及其在胃癌组织中的应用[J].分析化学,2011,39(12):1817-1822,6.基金项目
本文系国家自然科学基金(Nos.20775089,30970312)资助项目 （Nos.20775089,30970312）
