分析化学2011，Vol.39Issue(12)：1858-1863,6.DOI:10.3724/SP.J.1096.2011.01858
高效液相色谱-静电场轨道阱质谱鉴定大鼠血浆中蟾蜍二烯内酯及其代谢物
Identification of Bufadienolides and Its Metabolites in Rat Plasma by High Performance Liquid Chromatography Coupled with Linear Trap Quadrupole-Orbitrap Mass Spectrometry
摘要
Abstract
A method based on high performance liquid chromatography coupled with Linear trap quadrupole(LTQ)-Orbitrap mass spectrometry ( HPLC-LTQ-Orbitrap-MS) was developed for the identification of the bufadienolides and its metabolites in rat plasma. The rat plasmas were collected after oral administration of lactones from ChanSu extract (50 mg/kg). The plasma samples were precipitated by acetonitrile and concentrated, and then analyzed by HPLC-LTQ-Orbitrap-MS. 24 bufadienolides and the 20 metabolites were identified according to their elution orders in C18 column, exact molecular weight and MS/MS fragmentation provided by high-resolution MS/MS. 5 standards of bufadienolides were used for corroboration. The method is fast, sensitive and suitable to identify trace bufadienolides and its metabolites in plasma.关键词
蟾酥/蟾蜍二烯内酯/代谢物/血浆/高效液相色谱/静电场轨道阱质谱法Key words
Chansu/ Bufadienolides/ Metabolite/ Plasma/ High performance liquid chromatography/ Linear trap quadrupole-orbitrap-mass spectrometry引用本文复制引用
陈建新,邓洁薇,陈红,田海妍,杨运云,叶文才..高效液相色谱-静电场轨道阱质谱鉴定大鼠血浆中蟾蜍二烯内酯及其代谢物[J].分析化学,2011,39(12):1858-1863,6.基金项目
本文系国家自然科学基金面上项目(No.30972217)及重大研究计划项目(No.90913020)资助 （No.30972217）
