分析化学2012，Vol.40Issue(1)：89-94,6.DOI:10.3724/SP.J.1096.2012.10181
流动相的pH值和离子强度对牛血清蛋白高效液相色谱手性柱分离性能的影响
Effect of Ionic-Strength and pH Value in Mobile Phase on EnantioSeparation of BSA High Performance Liquid Chromatography Column
摘要
Abstract
BSA chiral columns were prepared based on column modification method with carbonyl imidazole as cross-linker. Enantioseparation of tryptophan, pidotimod and 4-phenyl-l,3-oxazolidine-2-thione (L-ben) was obtained on the BSA chiral column by HPLC. The effects of pH values and ionic strength of mobile phase on enantioseparation performance were investigated. For L-tryptophan, the retention factor (k') increased significantly as the pH value increased from 5. 0 to 7. 0. While for D-tryptophan k' rarely changed and the resolution factor (Rs) increased froml. 15 to 8. 91. In contrast, the retention factor (k ) for RS- and SR-pidotimod decreased as the pH value increased from 5. 0 to 7. 0. For L-ben, k' was only a slight increase when the pH value increased. The retention factors of three enantiomers fluctuated as ionic strength increased. However, Rs decreased when a buffer concentration increased.关键词
牛血清蛋白(BSA)/高效液相色谱/手性分离/pH值/离子强度Key words
Bovine serum albumin/High performance liquid chromatography/Chiral separation/pH value/Ionic strength引用本文复制引用
熊雅进,苏文翠,章伟光,范军,郑盛润,林纯..流动相的pH值和离子强度对牛血清蛋白高效液相色谱手性柱分离性能的影响[J].分析化学,2012,40(1):89-94,6.基金项目
本文系2010科技部科技型中小企业技术创新基金(No.10C26214412704)、广东省国际合作-重点引进项目(No.20101B050300021)和广东省教育部产学研结合项目(No.2009B090300081)资助 （No.10C26214412704）
