分析化学2012，Vol.40Issue(1)：124-128,5.DOI:10.3724/SP.J.1096.2012.10526
纳米纤维固相萃取柱萃取-高效液相荧光法分析头发皮质醇
Nanofibers Solid Phase Extraction Column for Determination of Cortisol in Hair Samples
摘要
Abstract
In recent years, many studies found that hair cortisol can serve as a good biomarker of chronic stress. A high performance liquid chromatography-fluorescence (HPLC-Flu) method for the determination of hair cortisol based on novel packed nanofiber solid phase extraction (PFSPE) was established. The incubation solution of hair samples was extracted by the new PFSPE column. The column was eluted with 100 μL ethanol, and then 230 μL concentrated sulfuric acid was add in the eluant and retained for 2 min. The mixture of reactants and water was secondly extracted by the PFSPE column, which was eluted with 50 μL methanol directly. The detection limit of cortisol was 0.5 ng/g(signal-to-noise ratio of 3). The within-run and between-day precisions (RSD) were below 6.8% and 8. 9%, and the average recovery of cortisol spiked into hair samples was 88. 9%. The method of pretreatment is simple, fast and green. Moreover, the established method can accurately determine the level of cortisol in hair samples, which provides a simple, inexpensive determination method for physiology, psychiatry, sports medicine, cognitive science, psychology and other researches.关键词
纳米纤维/头发/皮质醇/皮质酮/高效液相色谱/荧光检测Key words
Nanofiber/Hair/Cortisol/Corticosterone/High performance chromatography/Fluorescence引用本文复制引用
陈利琴,康学军,晏嫣,顾忠泽,陆祖宏..纳米纤维固相萃取柱萃取-高效液相荧光法分析头发皮质醇[J].分析化学,2012,40(1):124-128,5.基金项目
本文系国家"973"基金项目(No.2007CB936300),江苏省工业支撑项目资助(No.SBE201000676),苏州市科技计划资助(Nos.SYJG0912,SYN201006,SG201028)和东南大学优秀博士论文基金资助 （No.2007CB936300）
