分析化学2012，Vol.40Issue(2)：268-272,5.DOI:10.3724/SP.J.1096.2012.10633
亲和毛细管电泳法研究锌离子与人血清白蛋白的结合反应机制
Binding Interaction Between Zinc Ion and Human Serum Albumin Using Affinity Capillary Electrophoresis
摘要
Abstract
The interaction between metal ions and human serum albumin (HAS) was investigated using affinity capillary electrophoresis (ACE) method. A model about the interaction between zinc ion as the ligand and HAS as the receptor was established under physiological conditions. The changes of effective mobility were determined for this interaction system with N,N-dimethylformamide (DMF) as internal marker. Based on the Scatchard model, a practical nonlinear simulation equation was used to calculate the apparent binding constant and the interaction binding strength between metal ion and HAS was quantificational characterized, and a conclusion about a quick balance system of the interaction between metal ion and HAS was acquired by analyzing electropherogram. It was concluded that ACE method was simple and effective and the obvious response relationship existed between the interaction strength and the concentrations of zinc ion. This work has referential meaning for understanding deeply the interaction strength between metal ion and HAS in vivo. The affinity capillary electrophoresis method can be considered valid for studying the interaction between metal ions and protein molecules.关键词
亲和毛细管电泳/人血清白蛋白/锌离子/有效淌度/表观结合常数Key words
Affinity capillary electrophoresis/ Human serum albumin/ Zinc ion/ Effective mobility/ Apparent binding constant引用本文复制引用
郭明,刘咪咪,李铭慧,何玲..亲和毛细管电泳法研究锌离子与人血清白蛋白的结合反应机制[J].分析化学,2012,40(2):268-272,5.基金项目
本文系国家自然科学基金项目(No.20877072)和浙江省科技创新活动计划项目(No.2010R412042)资助 （No.20877072）
