分析化学2012，Vol.40Issue(2)：286-291,6.DOI:10.3724/SP.J.1096.2012.10463
高效液相色谱-串联质谱法测定蔬菜和水果中氟吗啉的残留量
Determination of Flumorph Residues in Vegetables and Fruits by High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method was developed for the determination of flumorph residues in vegetables and fruits using dimoxystrobin as internal standard (IS) by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) . The conversion rule between flumorph(Z) and flumorph(E) has been studied by sunlight test of flumorph. Flumorph and IS were extracted with ethyl acetate, then purified and concentrated by Oasis HLB cartridge. Qualitative and quantitative detection for the ana-lytes were carried out under the multiple reaction monitoring (MRM) in positive ion mode after the chromatographic separation on Symmetry C18(150 mm×2. 1 mm I. D. , 3. 5 μm) column. Studies at fortification level of 0. 05 - 25 μg/kg gave mean recoveries from 77. 6%-92. 8% for flumorph, with relative standard deviation (R. S. D. ) ≤8. 6%. The limit of quantification (LOQ, S/N=10) was 0. 05 fig/kg. The proposed method was successfully applied to real samples from different kinds of vegetables and fruits. Flumorph residues were detected in 70% of the samples analyzed and the highest concentration level was 1. 83 μg/kg in tomato sample.关键词
高效液相色谱-质谱/转化/氟吗啉/蔬菜/水果Key words
Liquid chromatography-tandem mass spectrometry/ Conversion/ Flumorph/ Vegetables/ Fruits引用本文复制引用
王岩松,杨春晖,张春野,张凤清,贺明睿,崔相勇,马吉,李华锋..高效液相色谱-串联质谱法测定蔬菜和水果中氟吗啉的残留量[J].分析化学,2012,40(2):286-291,6.基金项目
本文系辽宁省重点实验室项目(No.2008S093)资助 （No.2008S093）
