分析化学2012，Vol.40Issue(2)：317-320,4.DOI:10.3724/SP.J.1096.2012.10828
离子液体双水相溶剂浮选法分离/富集桑黄黄酮类成分
Separation/Richment Total Flavonoids in Phellinus Igniarius by Ionic Liquid/Salt Aqueous Two-phase Flotation
摘要
Abstract
Ionic liquid/salt aqueous two-phase flotation coupled with UV spectrophotometry was developed for the separation/enrichment and analysis of total flavonoids in Phellinus igniarius(Sanghuang). The method was combined with ionic liquid aqueous two-phase system based on ionic liquid (l-butyl-3-methylimidazolium chloride, [O6mim]CD and inorganic salt ( K2 HPO4 ) with solvent sublation. The effect on the flotation efficiency was studied. The best conditions were as follows: the concentration of K2 HPO4 was 50 %; pH of solution was 9. 53; the amount of [Qmin]Cl was 3 mL; the flotation time was 50 min; the nitrogen flow rate was 30 mL/min. The flotation efficiency was 85. 31 %, the enrichment factor was 8. 59. These values are higher and larger than those of two-phase extraction. The method is suitable for the separation and enrichment of the active ingredient of Chinese medicine.关键词
溶剂浮选/紫外分光光度法/离子液体/双水相/桑黄/总黄酮Key words
Solvent flotation/ Aqueous/ two-phase/ Ionic liquid/ Phellinus igniarius/ Total flavonoids引用本文复制引用
戈延茹,潘如,傅海珍,吴云,付文艳,闫永胜,戚雪勇..离子液体双水相溶剂浮选法分离/富集桑黄黄酮类成分[J].分析化学,2012,40(2):317-320,4.基金项目
本文系江苏大学高级人才引进专项资助项目(No.1283000138)和江苏省普通高校研究生科研新计划(No.CXLX11_0600)资助 （No.1283000138）
