法医学杂志2012，Vol.28Issue(1)：36-40,43,6.DOI:10.3969/j.issn.1004-5619.2012.01.009
X染色体上16个STR基因座的分型检测和多态性分析
Typing and Polymorphism Analysis of 16 STR Loci on X Chromosome
摘要
Abstract
Objective To develop a PCR-based X-STR kit for typing of 16 X-STR loci and investigate the polymorphisms of the X-STR markers. Methods Sixteen STR loci {GATA165B12, DXS101, GATA172D05, HPRTB, DXS981, DXS8378, DXS6795, GATA31E08, DXS6809, DXS6803, DXS9902, DXS6807, DXS7423, DXS7133, DXS6810 and DXS7132) located on X chromosome were selected. The primers for multiplex PCR were designed by Primer Premier 5.0 software and labeled by four fluorescences (FAM, HEX, TAM-RA and ROX). The developed multiplex PCR system was used for investigating the polymorphisms of the X-STR markers in Han populations. Results The 16-plex amplification system named Idtyper X-16 was successfully developed and validated. Among the 16 X-STR loci, DXS7133 and DXS7423 were found to be moderately polymorphic and the other 14 X-STR markers were highly polymorphic (PIC>0.5, H>0.5). The cumulative discrimination power in females and in males were 0.999999 999 99997 and 0.999 999993 respectively in Han population. The combined power of exclusion in trios and in duos were 0.999 999 93 and 0.999 990, respectively. Conclusion The Idtyper X-16 kit is highly valuable in forensic science and is suitable for paternity testing in disputed cases.关键词
法医遗传学/X染色体/多态现象,遗传/STRKey words
forensic genetics/ X chromosome/ polymorphism, genetic/ STR分类
社会科学引用本文复制引用
李莉,赵书民,张素华,李成涛,柳燕,林源,刘俊宏..X染色体上16个STR基因座的分型检测和多态性分析[J].法医学杂志,2012,28(1):36-40,43,6.基金项目
上海市科学技术委员会科研计划项目(08251203200) （08251203200）
上海市自然科学基金项目(08ZR1419800) （08ZR1419800）
上海市法医学重点实验室资助项目(11DZ2271500) （11DZ2271500）
