亚热带农业研究2011,Vol.7Issue(3):197-200,4.
酿酒酵母ADH2基因沉默表达载体的构建
Constructing the alcohol dehydrogenase ADH2 gene silencing expression vector for Saccharomyces cerevisiae
摘要
Abstract
It was to synthesize the silencing gene SADH2 of Saccharomyces cerevisiae with overlapping PCR.The silencing gene SADH2 was inserted in pYES2.0 to construct S.cerevisiae ADH2 gene silencing expression vector.Sequencing results showed that the expression vector pSADH2 was correct.关键词
酿酒酵母/乙醇脱氢酶/pYES2.0/重叠延伸PCRKey words
Saccharomyces cerevisiae/alcohol dehydrogenase/pYES2.0/overlap PCR分类
生物科学引用本文复制引用
林燕环,魏芳,叶冰莹,张积森,陈由强..酿酒酵母ADH2基因沉默表达载体的构建[J].亚热带农业研究,2011,7(3):197-200,4.基金项目
现代农业产业技术体系建设专项资金资助 ()