高等学校化学学报2012，Vol.33Issue(1)：49-53,5.DOI:10.3969/j.issn.0251-0790.2012.01.008
微流控细胞芯片LED诱导荧光检测微系统
Microfluidic Cell Chip Analytical System with LED Induced Fluorescence Detector
摘要
Abstract
Based on micro-electro-mechanical systems technique and microfluidic cell chip analytical methodology, a microfluidic cell chip analytical system with light emitting diode (LED) induced fluorescence detector was constructed for monitoring cells in the suspensions with a low concentration below 40 Cell/mL. Cells passed through the micro-channels on the designed microchip, and the target cells labeled by 5- or 6-(N-suc-cinimidyloxycarbonyl)-3',6'-0, O'-diacetylfluorescein(CFDA SE) were stimulated by the LED to produce fluorescence signals which were collected by a photomultiplier tube from the roof window and changed into electrical signals finally. The HepC2 hepatoma cells labeled by CFDA SE in samples could be detected and counted by this analytical microsystem. The designed microsystem ran effectively with the coexistence of blood cells. The whole microsystem is simple, convenient and high sensitivity.关键词
微流控芯片/HepG2肝癌细胞/细胞检测/发光二极管(LED)诱导荧光检测微系统Key words
Microfluidic chip/ HepG2 hepatoma cell/ Cell detection/ Microsystem with light emitting diode(LED) induced fluorescence detector分类
化学化工引用本文复制引用
李栋顺,徐溢,彭金兰,周桢,甘俊,王尧..微流控细胞芯片LED诱导荧光检测微系统[J].高等学校化学学报,2012,33(1):49-53,5.基金项目
全国博士学位论文作者专项资金(批准号:200941)、国家自然科学基金(批准号:20675089)、国家科技部"创新药物孵化基地"子课题(批准号:2010ZX09401-306-1-5)、重庆大学研究生科技创新基金(批准号:CDJXS10-22-11-39)和中央高校基本科研业务费专项资金(批准号:CDJXS1-22-00-05)资助. （批准号:200941）
