高等学校化学学报2012，Vol.33Issue(2)：308-312,5.DOI:10.3969/j.issn.0251-0790.2012.02.017
海地瓜蛋白水解物中ACE抑制肽的分离纯化及合成
Purification and Synthesis of ACE Inhibitory Peptide from Acaudina molpadioidea Protein Hydrolysate
摘要
Abstract
A novel ACE inhibitory peptide was isolated from the sea cucumber (Acaudina molpadioidea) hydrolysate using the chromatographic methods including gel filtration, ion-exchange chromatography and reversed phase high-performance liquid chromatography. The purified ACE inhibitory peptide was sequenced as MEGAQEAQGD, with a IC50 value of 15.9 μmol/L. The ACE inhibitory peptide was synthesized by a method of gradual condensation and fragmental condensation. The purity of the decapeptide was 99. 72% , and the molecular weight and sequence structure of the decapeptide equate with the fact. It was found that the inhibitory activity of the peptide was intensified by 3. 5 times after incubation with pepsin and chymotrypsin. The ACE inhibitory peptide from Acaudina molpadioidea showed a clear antihypertensive effect on spontaneously hypertensive rats(SHR), at a dosage of 3 μmol/kg.关键词
海地瓜/ACE抑制肽/分离纯化Key words
Acaudina molpadioidea/ ACE inhibitory peptide/ Isolation and purification分类
化学化工引用本文复制引用
赵元晖,李八方,马敬军,董士远,刘尊英,曾名湧..海地瓜蛋白水解物中ACE抑制肽的分离纯化及合成[J].高等学校化学学报,2012,33(2):308-312,5.基金项目
国家自然科学基金(批准号:31000830)和山东省自然科学基金(批准号:ZRR2010CQ001)资助. （批准号:31000830）
