高等学校化学学报2012，Vol.33Issue(2)：389-394,6.DOI:10.3969/j.issn.0251-0790.2012.02.031
明胶/聚乳酸复合纤维膜的制备、表征及作为角膜细胞载体的评价
Preparation and Characterization of Gelatin/PLLA Composite Fibrous Scaffold and Evaluation for Corneal Cells Supports
摘要
Abstract
Gelatin and polylactic acid (PLLA) composite membranes were obtained via electrospun. The membranes composition on the surface properties, pore structure and mechanical properties were studied. The membranes were further used as scaffolds for rabbit corneal epithelial cells culture in vitro. The adhesion and proliferation of cells on these scaffolds were evaluated by immunofluorescence staining and thiazolyl bule tetrazolium bromide (MTT) assay. The results show that the fiber diameter distribution and surface hydrophi-licity are significantly affected by composition of electrospinning solution. All of these scaffolds have high porosity and inter-connected pores. Gelatin could maintain high cell adhesion while PLLA improved mechanical properties of the composited scaffolds.关键词
明胶/聚乳酸/复合支架/角膜细胞Key words
Gelatin/ Poly-L-lactic acid/ Composite scaffold/ Corneal cell分类
化学化工引用本文复制引用
颜静,高岩,王清,崔学军,钟双玲,王萍,王洪艳..明胶/聚乳酸复合纤维膜的制备、表征及作为角膜细胞载体的评价[J].高等学校化学学报,2012,33(2):389-394,6.基金项目
国家自然科学基金(批准号:50673032)和吉林大学研究生创新基金(批准号:20111035)资助. （批准号:50673032）
