肝胆胰外科杂志2012，Vol.24Issue(1)：57-59,3.
INF-γ对肝纤维化大鼠TGF-β1/Smad3蛋白表达的影响
Effect of INF-γ on hepatic fibrosis in rats of TGF-β1/Smad3 protein expression
摘要
Abstract
Objective To observe the INF- γ on hepatic fibrosis and changes of TGF- β 1> Smad3 protein expression, and explore the mechanism of INF- γ in treating hepatic fibrosis. Methods Male SD rats were divided into three groups, model group and experimental group were injected with thioacetamide (thioacetamide, TAA) 100 mg/kg, 2 days by intraperitoneal injection to induce rat hepatic fibrosis, The control grounp was injected with equal amount of physiological saline, while the experimental group treated with INF- γ intramuscularly .Model group and control group injected with equal physiological saline. All rats were sacrificed at 8 weeks ,stained with Masson to observate hepatic fibrosis, detected TGF- β 1, Smad3 protein levels with immunohistochemistry in hepatic tissue. Results The degree of experimental group hepatic fibrosis, the expression of TGF- β 1, Smad3 was higher than that in the normal group (P<0. 05), significantly lower than that in the model group (P<0.05). Conclusion IFN- γ may inhibit TGF- β 1/Smad3 protein expression, and thus play a role in hepatic fibrosis.关键词
肝纤维化/干扰素γ/转化生长因子β1/Smad3/信号转导Key words
hepatic fibrosis/ INF- γ / TGF- β 1/ Smad3 / signal transduction分类
医药卫生引用本文复制引用
黄越龙,周春光,张谊,张启瑜,汪海清,裴小龙..INF-γ对肝纤维化大鼠TGF-β1/Smad3蛋白表达的影响[J].肝胆胰外科杂志,2012,24(1):57-59,3.基金项目
浙江省科技厅资助项目(2010R413034). （2010R413034）
