广东医学2012，Vol.33Issue(4)：439-441,3.
和厚朴酚对小鼠黑色素瘤B16细胞增殖以及黑色素合成的影响
Inhibition of honokiol on the proliferation and melanin biosynthesis on B16 melanoma cells in vitro
摘要
Abstract
Objective To investigate the inhibition of honokiol on proliferation and melanin biosynthesis in B16 melanoma cells in vitro. Methods B16 cells were cultured in vitro. MTT assay, DAPI and NaOH lysis test were applied for assessment of cell proliferation, cellular morphologic observation and cellular melanin content, respectively. Results B16 cells were treated with honokiol with different concentrations ( 5,10,20,40 and 80 μmol/L ) and durations ( 12,24 and 48 h ). The IC50 of honokiol on proliferation of B16 cells were 23. 4, 13. 1 and 11.4 μmol/L, respectively, with durations of 12,24 and 48 h. Apoptosis of B16 cells were induced with honokiol in DAPI staining. Furthermore, the melanin biosynthesis was inhibited with honokiol with concentration over 20 (xmol/L. Conclusion Honokiol effectively inhibits the proliferation of B16 in dose - and time - depend manners. Apoptotic bodies were observed in B16 cells treated with honokiol. Although melanin biosynthesis is also inhibited by honokiol, this effect is insignificant.关键词
和厚朴酚/黑色素瘤B16/细胞增殖/黑色素合成Key words
honokiol/ B16 melanoma cell/ proliferation/ melanin biosynthesis引用本文复制引用
喻丽红,张超,谭茵..和厚朴酚对小鼠黑色素瘤B16细胞增殖以及黑色素合成的影响[J].广东医学,2012,33(4):439-441,3.基金项目
广东省科技计划项目(编号:2009B060700078),广州市属高校科技计划项目(编号:08A102) （编号:2009B060700078）
