广东医学2012，Vol.33Issue(6)：723-725,3.
原代小胶质细胞及神经元细胞培养不同纯化方法的比较
Comparison of different purified methods in culture of primary microglia and neuron culture
摘要
Abstract
Objective To establish an easy and effective method for culture and purification of primary microglia and neurons. Methods Mild trypsinization, mechanical shaking ( 37℃ 2 h at 250 r/min ), lidocaine hydrochloride and hand shaking methods were applied for isolation and purification of glias from mixed glias and neurons culture, while neurons with neurobasal medium and cytarabine dealing methods. All the purified cells were counted. The purity of isolated microglia and neuron were assessed by flow cytometriy and immunocytochemitry, respectively. Results The hand shaking method for isolation of microglias was proved with highest efficacy and vivacity, and so was the neurobasal medium method for neurons. Conclusion It's a productive and economic method of hand shaking method in purification of microglia, and so is neurobasal culture method for neurons.关键词
小胶质细胞/神经元/原代培养/纯化方法Key words
microglia/neuron/primariy culture/purification引用本文复制引用
王宝萍,李东风,徐书雯..原代小胶质细胞及神经元细胞培养不同纯化方法的比较[J].广东医学,2012,33(6):723-725,3.基金项目
广东省医学科研指令性课题(编号:C2009031),广州市科技计划项目(编号:2010Y1-C101) （编号:C2009031）
