干旱地区农业研究2012，Vol.30Issue(1)：138-142,148,6.
黑麦草幼苗对NaHCO3胁迫的生理响应
Physiological response of ryegrass seedlings to NaHCO3 stress
摘要
Abstract
Ryegrass seedlings were exposed to Hoagland's nutrient solution with NaHCO3 added (0, 50, 100, 150, 200 mmol/L), and the effects of NaHCO3 stress on the plant growth, metabolism of reactive oxygen species (ROS) and osmotica accumulation in leaves were studied by a sandy culture. The results showed (1) With the increasing of NaHCO3 concentration, the fresh and dry weights of the seedlings decreased, the contents of O2- , H2O2, MDA and Na+ in leaves increased gradually, and the activities of CAT and POD, the contents of AsA, GSH and K+ as well as K+/Na+ de-creased continually, and the activities of SOD and APX exhibited a humped-curve, while the activities of V - H+ - AT-Pase and V - H+ - Ppase were higher than the control at 50 ~ 150 mmol/L NaHCO3 but lower than the control at 200 mmol/L NaHCO3- (2) Alkali-stress decreased the contents of soluble protein and free amino acid while increased the contents of soluble sugar and proline in the seedling leaves. It was concluded that the imbalances of ROS metabolism and Na+ to K+ in the seedling leaves were possibly caused by alkaline stress, and consumed more energy for osmotic adjust-ment by accumulating organic solutes, which might be an important reason that the growth of ryegrass seedlings under al-kaline stress was inhibited.关键词
NaHCO3胁迫/黑麦草/活性氧代谢/生理响应Key words
NaHCO3 stress/ ryegrass/ ROS metabolism/ physiological responses分类
农业科技引用本文复制引用
刘建新,王鑫,王金成,李东波,王凤琴..黑麦草幼苗对NaHCO3胁迫的生理响应[J].干旱地区农业研究,2012,30(1):138-142,148,6.基金项目
甘肃省庆阳市科技攻关计划项目(0802NKCM082) （0802NKCM082）
