干旱地区农业研究2012，Vol.30Issue(1)：184-189,6.
秦岭山区无机磷细菌筛选及其Biolog和分子生物学鉴定
Isolation and identification of inorganic phosphorus-solubilizing bacteria by Biolog and molecular method
摘要
Abstract
A study was conducted to isolate effective inorganic phosphorus-solubilizing bacteria for potential research and production of biofertilizer. Three strains, named as NO.9, N015 and NO. 17, are isolated from soil samples collect-ed from Qinling Mountains by their dissolving phosphorus circle on PKO Agar media. After 5 days incubation, through Mo-Sb colorimetry, the soluble phosphorus content in liquid media is 98.12 mg/L, 80.37 mg/L and 104.91 mg/L re-spectively, and the content of microbial biomass phosphorus is 62.85 mg/L, 53.61 mg/L and 36.59 mg/L. Analysed by physiology and biochemistry experiments, Biolog and 16S rDNA sequencing reflects that all of the three strains are gram-negative intestinal bacteria, and NO.9 strain is identified as Inquilinus ginsengisoli, NO. 15 strain as Acinetobacter calcoaceticus and NO. 17 strain as Pseudomonas fluorescens.关键词
解磷细菌/鉴定/Biolog/16S rDNAKey words
inorganic phosphate-solubilizing bacteria/ identification/ Biolog/ 16S Rdna分类
农业科技引用本文复制引用
刘江,陈琳,邵丽,谷洁,高华,秦清军,陈胜男,李海龙,张卫娟,郭星亮,张洪宾..秦岭山区无机磷细菌筛选及其Biolog和分子生物学鉴定[J].干旱地区农业研究,2012,30(1):184-189,6.基金项目
国家自然科学基金(40771109,40871119) （40771109,40871119）
