果树学报2011，Vol.28Issue(5)：808-813,6.
CjFRO2在转基因枳中的整合与表达
Studies of the integration and expression of CjFRO2 gene in transgenic Poncirus trifoliata
摘要
Abstract
Iron is an essential trace element for plants,and it is well known that iron deficiency is a significant global problem.Fruit trees are perennial plants,have been more seriously affected by iron deficiency.Breeding for tolerance to iron deficiency using conventional breeding and selection techniques is very time-consuming,laborious,and inefficient.Genetically modifying fruit trees to improve iron absorption is a complementary approach.Ferric chelate reductase（FCR） is a rate-limiting enzyme in iron uptake of plant,plays an important role in iron homeostasis.Citrus junos is characterized by its tolerance to iron deficiency in alkaline and calcareous soils,the gene of Citrus junos ferric chelate reductase（CjFRO2） show strong expression in root tip,reductase activities are greatly increased.Poncirus trifoliata is the predominant citrus rootstock in China,but it is highly sensitivity to iron deficiency.In order to enhance iron uptake ability,the gene of CjFRO2 driven by CaMV 35S promoter was introduced into the Poncirus trifoliata via Agrobacterium-mediated transformation.The primary transgenic plants were screened using PCR（polymerase chain reaction）,Southern hybridization for detection of transgenic integrations and Real-Time for investigation CjFRO2 gene expression.Four independent single copy transgenic lines were obtained and showed constitutive expression of CjFRO2,and the expression of transgenes after vegetative propagation was highly stable.关键词
香橙/三价铁螯合物还原酶基因/枳/Southern杂交分析/Real-TimePCR表达分析Key words
Citrus junos/Ferric-chelated Reductase/Poncirus trifoliata/Southern blot analysis/Real-Time PCR analysis分类
农业科技引用本文复制引用
刘小丰,彭爱红,许兰珍,雷天刚,姚利晓,何永睿,陈善春..CjFRO2在转基因枳中的整合与表达[J].果树学报,2011,28(5):808-813,6.基金项目
长江学者和创新团队发展计划项目(PCSIRT) （PCSIRT）
国家高科技研究发展计划项目(863)(2011AA100205) （863）
柑桔产业技术体系(CARS-27) （CARS-27）
农业部"引进国际先进农业科学技术"项目("948"计划) （"948"计划）
中央高校基本科研业务费专项(XDJK2009C136) （XDJK2009C136）
