果树学报2011,Vol.28Issue(5):814-819,6.
广西龙荔遗传多样性的ISSR分析
Genetic diversity analysis of Dimocarpus confinis in Guangxi by intersimple sequence repeats(ISSRs)
摘要
Abstract
Twelve pairs of ISSR primer combinations were used to determine the genetic diversity of Dimocarpus confinis in Guangxi.Materials included 67 D.confinis individual plants,10 longan varieties,12 litchi varieties and 10 wild litchi plants in Guangxi and all the materials obtained clear fingerprints.A total of 151 major ISSR bands were observed,and 127 bands(84.11%) were polymorphic.All of the cultivars could be distinguished with the markers used.Genetic similarities among all D.confinis ranged from 0.61 to 0.97,and it indicated that D.confinis germplasm in Guangxi had abundant genetic diversity.There were obvious differences among D.confinis,longan and litchi,genetic similarities among all 99 taxa ranged from 0.53 to 0.98,litchi formed a single cluster and D.confinis and longan formed another cluster at Dice coefficient 0.53.The results showed that D.confinis was closer to longan in genetic material.关键词
龙荔/遗传多样性/ISSR/广西Key words
Dimocarpus confinis/Genetic diversity/ISSR/Guangxi分类
农业科技引用本文复制引用
潘丽梅,朱建华,秦献泉,彭宏祥,黄凤珠,卢美英..广西龙荔遗传多样性的ISSR分析[J].果树学报,2011,28(5):814-819,6.基金项目
广西农业科学院基本科研业务专项(201035基) (201035基)
广西科学基金项目(桂科自0339023) (桂科自0339023)