果树学报2011，Vol.28Issue(6)：1038-1044,F0003,8.
重组ALA合酶基因（YHem1）转化草莓提高叶片光合能量转化能力的效应
Effect of transformed recombinant ALA synthase gene （YHem1） on leaf photosynthetic energy conversion in strawberry
摘要
Abstract
In order to breed new varieties with higher photosynthetic capacity under stress conditions, the experiments were carried out to establish regeneration system from leaf explants of Benihonppe strawberry （Fragaria ananassa Duch.） and then a recombinant gene YHeml, yeast ALA synthase gene controlled by Arabidopsis thaliana HemAl gene promoter, was transformed into the detached leaves mediated with A- grobacterium tumefaciens. After kanamycin selection, PCR and RT-PCR detection, three plants were i- dentified to be the transgenic plants. The chlorophyll fast fluorescence showed that the transgenic straw- berry remained higher leaf maximal photochemical efficiency （F、/Fm）, photosynthetic performance index （PI_ABS） and probability of a trapped exciton moving an electron into the electron transport chain beyond QA- （ψ.）, while the approximate initial slope of the fluorescence transient （Mo） was lower in transgenie plants than in the wild type. This suggested that Yheml transformation might increase the capacity of photosyn- thetic energy conversion in strawberry leaves.关键词
红颊草莓/重组ALA合酶基因（YHem1）/遗传转化/转基因植株/叶绿素荧光Key words
Benihoppe strawberry/Recombinant ALA synthase gene （YHem1）/Genetic transformation/Transgenic plants/Chlorophyll fast fluorescence分类
农业科技引用本文复制引用
林晓慧,张治平,韩丽晓,吴震,汪良驹..重组ALA合酶基因（YHem1）转化草莓提高叶片光合能量转化能力的效应[J].果树学报,2011,28(6):1038-1044,F0003,8.基金项目
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
