果树学报2011，Vol.28Issue(6)：1059-1062,I0004,5.
薄皮甜瓜白啄瓜枯萎病抗性基因Fom-2的同源克隆与表达分析
Cloning and transcriptional analysis of resistance gene Fom-2 from Cucumis melo ssp. Agrestis Jeffrey
摘要
Abstract
Fusarium wilt （Fusarium oxysporum f. sp. melonis Snyder and Hansen） is one of destructive disease in melon crop production. Fore-2 gene was genetically identified to control the resistance of races 0 and 1. Resistance to fusarium wilt breeding is one of important targets for melon breeding. In this study, we cloned the Fore-2 gene from Baizhuogua melon （Cucumis melo ssp. agrestis Jeffrey） by using homo- logical cloning method, which was deduced to encode 1099 amino acids and exhibited high similarity to NBS-LRR type of R genes in plant. The deduced FOM-2 protein contained NB-ARC and SCOP typical motifs of R gene. The expression of Fore-2 gene was highly induced after inoculation with fore race 1. In eonclusion, the cloning of Fore-2 gene is good for breeding with resistance to fusarium wilt.关键词
薄皮甜瓜/自啄瓜/枯萎病/Fom-2基因Key words
Cucumis melo/Baizhuogua/Fusarium wilt/Fom-2 gene分类
农业科技引用本文复制引用
杨加付,赵宣,刘菊,杨晓东,王士伟,杨景华,张明方..薄皮甜瓜白啄瓜枯萎病抗性基因Fom-2的同源克隆与表达分析[J].果树学报,2011,28(6):1059-1062,I0004,5.基金项目
浙江省自然科学基金项目(Y3080084) （Y3080084）
浙江省科技计划项目(2008C22060) （2008C22060）
