果树学报2011，Vol.28Issue(6)：1082-1085,4.
甜樱桃B类MADS—box基因的分离与序列分析
Isolation and sequence analysis of B-class MADS-box gene in sweet cherry
摘要
Abstract
To study the role of MADS-box genes in floral development of sweet cherry, MADS-box gene was isolated from Prunus avium L. Lapins with RT-PCR and RACE approaches. Primers were designed according to the conservative region sequences of various plant MADS-box family genes, and semi-quan-titative PCR was used to analyze the expression of gene in different organs and tissues. The full length of cDNA is 962 bp, named PaMADS2 （the accession numbers in GenBank： HQ229606）, with an open reading frame （ORF） of 633 bp and encoding 210 amino acid residues. Sequence comparison and phylogenetic analysis indicated that PaMADS2 closely resembles the PI clade of class B. Tissue specific analysis showed that PaMADS2 expressed in petal and stamen. The results suggested that PaMADS2 belongs to the B-class MADS-box gene family.关键词
甜樱桃/花器官/雄蕊/花瓣/MADS—boxKey words
Sweet cherry （Prunus avium L. ）/Floral organs/Stamen/Petal/MADS box分类
农业科技引用本文复制引用
林苗苗,赵长竹,顾红,张慧琴,陈锦永,谢鸣,方金豹..甜樱桃B类MADS—box基因的分离与序列分析[J].果树学报,2011,28(6):1082-1085,4.基金项目
十一五科技支撑资助项目(2006BAD07806-10) （2006BAD07806-10）
浙江省科技计划面上项目(2009C32031) （2009C32031）
中国农业科学院果树生长发育与品质控制重点开放实验室资助项目(PP333KF20090101) （PP333KF20090101）
