果树学报2011，Vol.28Issue(6)：1099-1103,5.
基于16S rDNA序列比对分析赣南脐橙上柑橘黄龙病病原的分子特征
Molecular characterization of Citrus Huanglongbing Bacteria in Gannan navel orange based on 16S rDNA sequences alignment
摘要
Abstract
Seven Citrus Huanglongbing （HLB） bacteria of Gannan navel orange in Jiangxi Province were detected by PCR with primers specific to Asian, African and American liberibacters. The Xba I restriction endonucleases were used to digest the HLB 16S rDNA fragment of Gannan navel orange, and the sequences of 16S rDNA were sequenced and compared with those corresponding sequences of Asian, African and American liberibacters from GenBank by BioEidt and MEGA software. The results showed that the HLB pathogen of Gannan navel orange could be detected by the specific primers of Candiatus L. asiaticus. RFLP analysis demonstrated that the digested Xba I profiles of 16S rDNA of the HLB isolates from Gannan navel orange had similar profiles with Ca. L. asiaticus. The 16S rDNA sequences of the 7 HLB isolates have identity ranging from 99.8% to 100%, and 98.4% to 99.9% with Asian liberibacters were higher identity than those with African liberibacters or American liberibacters. The same results were found by analyzing the sequences average nucleotide distance of HLB isolates of Gannan navel orange, Asian, African and American liberibacters. Phylogenetic analysis revealed that the 7 HLB isolates of Gannan navel orange were located in different phylogenetic clusters with African and American liberibacters, and classified into one cluster with Asian liberibacters, and shared higher homology and closer relationships. The results indicated that the HLB isolates of Gannan navel orange were Ca L. asiaticus.关键词
赣南脐橙/柑橘黄龙病/16S/rDNA/RFLP分析/序列比对Key words
Gannan navel orange/Citrus Huanglongbing/16S rDNA/RFLP analysis/Sequence alignment分类
农业科技引用本文复制引用
易龙,声苦军,钟八莲,赖晓桦,陈慈相..基于16S rDNA序列比对分析赣南脐橙上柑橘黄龙病病原的分子特征[J].果树学报,2011,28(6):1099-1103,5.基金项目
江西省青年科学家培养计划(2010DQ02300) （2010DQ02300）
江西省科技支撑计划项目(2010BNB01402) （2010BNB01402）
江西省教育厅科技项目(GJJ11585),公益性行业(农业)科研专项经费(201003067-02-6) （GJJ11585）
