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黄河故道地区葡萄根结线虫rDNA—ITS—PCR研究

刘三军 于巧丽 李洪连

果树学报2011,Vol.28Issue(6):1104-1106,I0005,4.
果树学报2011,Vol.28Issue(6):1104-1106,I0005,4.

黄河故道地区葡萄根结线虫rDNA—ITS—PCR研究

Study on rDNA -ITS-PCR of root-knot nematode in grape in the Chinese Yellow River east region

刘三军 1于巧丽 2李洪连2

作者信息

  • 1. 河南农业大学植物保护学院
  • 2. 中国农业科学院郑州果树研究所,郑州450000
  • 折叠

摘要

Abstract

The amplification, cloning and sequencing of the rDNA-ITS region of 6 different populations of root-knot nematodes from trail of the Yellow River east region were conducted. The sequence analysis results showed that six populations of root-knot nematodes on grape from Luoyang, Zhengzhou, Kaifeng, Shangqiu in Henan Province and Dangshan, Xiaoxian in Anhui Province belong to Meloidogyne incognita. The length of ITS was 766 bp. The results of the sequence analysis and the form identifications showed that this pathogen was Meloidogyne incognita.

关键词

葡萄/根结线虫病/南方根结线虫/ITS—PCR/序列分析

Key words

Grape/Root-knot nematode disease/Meloidogyne incognita/ITS-PCR/Sequence analysis

分类

农业科技

引用本文复制引用

刘三军,于巧丽,李洪连..黄河故道地区葡萄根结线虫rDNA—ITS—PCR研究[J].果树学报,2011,28(6):1104-1106,I0005,4.

基金项目

国家葡萄产业技术体系(cyjstx-30) (cyjstx-30)

果树学报

OA北大核心CSCDCSTPCD

1009-9980

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