果树学报2011，Vol.28Issue(6)：1111-1116,6.
猕猴桃EST-SSR引物筛选及通用性分析
Mining and transferability analysis of EST-SSR primers in Kiwifruit （Actinidia spp.）
摘要
Abstract
ESTs of Actinidia in the NCBI database were downloaded and screened by SSRHunter software, the EST-SSR primers were designed by Primer 5.0, and their transferabilities were analyzed in some Citrus plants. The 97 EST-SSR primers which were deigned were mined by using genomic DNA of Actinidia lijiangensis. The results indicated that the 77 pairs of primer showed amplification, accounting for 79.38%. Twenty pairs of primer selected were detected to PCR for DNAs from 10 A ctinidia varieties, the 17 primer pairs of the 20 showed amplification and polymorphism, accounting for 85%. The transferability of 20 pairs of EST-SSR primers which were randomly selected was explored in 9 Citrus germplasms （Okit-su, Kumquat, Trifoliate Orange, Ponkan, Sour Pummelo, Sweet Orange, Huyou, Owari, Miyagawa）. The results showed that the 11 primer pairs of the 20 tested primers had the amplification, accounting for 55%. And the 8 primer pairs showed polymorphism, accounting for 72.7%. The results revealed that the EST-SSR markers in A ctinidia were transferable in some Citrus germplasms.关键词
猕猴桃/EST-SSR/引物筛选/柑橘/通用性Key words
Kiwifruit （A ctinidia）/EST-SSR/Mining of primers/Citrus/Transferability分类
农业科技引用本文复制引用
廖娇,黄春辉,辜青青,曲雪艳,徐小彪..猕猴桃EST-SSR引物筛选及通用性分析[J].果树学报,2011,28(6):1111-1116,6.基金项目
国家自然科学基金(No.30660113,30860167) （No.30660113,30860167）
