摘要
Abstract
Objective To develop a simple and rapid RP-HPLC method for simultaneously determination of polydatin,resveratrol,emodin and physcion in extract from Polygonum cuspidatum. Methods The HPLC method was performed on a Kromasil Cs at 30 ℃, and the mobile phase was methanol-water with gradient elution at a flow rate of 1 mL/min. The detection wave length was 290 run, and 20 μL was injected. Results The linear range of polydatin,resveratrol,emodin and physcion were 10.54 -52.7μ,g/mL,4. 5 -22.5 μg/mL,4.92 -49. 2μg/mL and 3.51 -35. 1μg/mL,respectively. The average recovery ratio were 97. 70% ,97. 20% , 102.6% and 100.0% ,with the relative standard deviations(RSD)of 2.36% , 1. 89% , 1.23% and 3. 67% , respectively. Conclusion The method proved to be rapid, accurate and simple, suitable for the quality control of extract of Polygonum cuspidatum.关键词
高效液相色谱法/虎杖/白藜芦醇苷/白藜芦醇/大黄素/大黄素甲醚Key words
RP-HPLC/ Polygonum cuspidatum/ polydatin/ resveratrol/ emodin/ physcion分类
药学
