国际妇产科学杂志2011，Vol.38Issue(6)：520-523,540,5.
解脲支原体血清型3和8在母兔生殖道中的致病性研究
Study on Pathogenicity of Ureaplasma Urealyticum Serotype 3 and 8 in Female Rabbits' Reproductive Tract
摘要
Abstract
Objective:To study the pathogenicity of U reaplasma urealytioum serotype 3(Uu3) and 8(Uu8) with the same concentration in Female Rabbits, s Reproductive tract. Methods: Fifty-two oestradiol-treated adult female rabbits were randomly divided into group A, B, C and D. Ureaplasma urealyticum serotype 3,8,3 and 8, with the same concentration of l06 copy/g while 4 rabbits of control group(group D) inoculated sterile medium.They were detect Uu from cervical secretions by fluorescent quantitative polymerase chain reaction (FQ-PCR) at 1,3,7, 14, 21, 35,49,63,77,91,105 days. They were necropsied at 14,105 days of postinoculation randomly to observing tissues of cervix,endometria,fallopian tube by light microscope and cervical mucosa by scanning electron microscope. Results: ①The total positive rabbits of Uu in three experimental groups show a significant difference (P<0.05),which were 27.3%(25/88) ,55.7%(49/88) ,69.3%(61/ 88). But before 35 days,the positive rate of the three experimental groups at the same time point in all of Female rabbits infected cervical secretions does not show a statistically significance (P> 0.05) ,and the positive rate descended as the days of postinoculation lasting. The highest positive rates of three experimental groups were 87.5% (7/8), 100.0% (8/8), 100.0% (8/8) at 1 d after inoculation, but the conlisation didn't persist at 105 d. All rabbits of control group were negative. ②There was not statistical difference in the quantity of Uu among experimental groups (P>0.05). Their peak level were 364x 104copy/g,401 xl04copy/g,493xl04copy/g,reached at Id,but the FQ-PCR values reduced as the days of postinoculation lasting.In the same experimental group at different time points,the concentrations was a significant difference (P< 0.05).③ The total pathogenicity rates of three groups were significantly different (P=0.015),which were 31.3%(5/16) ,62.5%(10/ 16) ,81.3%(13/16). But there were not statistical difference among experimental groups at 14 days(P>0.05),but 105 days later the pathogenicity rates of three groups were significant different (P=0.041). None of control group was positive. Conclusions : ①in female rabbits's reproductive tract, the pathogenicity of each experimental group was shown when inoculatin Uu with FQ-PCR value is 106copy/g. the pathogenicity rates of three groups were significantly different, the pathogenicity of the mixed group (group C) was the highest and Uu3 group (group A) the lowest, Uu8 group (groupB) was the Second. ②In Female Rabbits,s Reproductive tract,the infectivity and quantatitive value were significant different among experimental groups,the infectivity and quantatitive value of the mixed group (group C) was the highest and Uu3 group (group A) the lowest,Uu8 group (group B) was the Second when inoculating Uu with the same concentration. ③The pathogenic concen-tration of Uu3 and Uu8 may differ, Uu8 may be < l06 copy/g, so further research is needed confirm it.关键词
解脲支原体/血清分型/聚合酶链反应/毒力/模型,动物Key words
Ureaplasma urealyticum/ Serotyping/ Polymerase chain reaction/ Virulence/ Models, animal引用本文复制引用
刘淑荣,张洪文,吴献青,王新,周英,李菡..解脲支原体血清型3和8在母兔生殖道中的致病性研究[J].国际妇产科学杂志,2011,38(6):520-523,540,5.基金项目
湖南省2011科技基本建设项目(20111318) （20111318）
