摘要
Abstract
Objective:To evaluate the application of Thin-Prep Cytology test(TCT), human papillomavirus (HPV) DNA、human telomerase RNA component(hTERC)gene detection in cervical cancer screening. Methods:From December 2010 to March 2011 in Henan Province People's Hospital gynecology clinic 1000 cases,had TCT、HPV (SPR method) 、 hTERC detection (fluorescence in situ hybridization FISH technique )respectively,to screen the cervical carcinoma,the above three kinds of detection of any positive result underwent colposcopy and cervical biopsy, based on the histopathology as the gold standard to evaluate three methods of cervical screening sensitivity .specificity ,Youden index and the coincidence rate. ResultS:One thousand cases of patients,average age 41±9,TCT abnormal results were 119 cases,accounting for 11.9%; HPV-positive 136 cases, accounting for 13.6%; hTERC gene amplification in a total of 58 positive cases, accounting for 5.8%. 229 routine colposcopy and cervical biopsy were done, in which 36 cases of cervical intraepithelial neoplasia (CIN), including 13 CIN I (5.68%),13 CINE (5.68%),8 CIN HI (3.49%),2 squamous cervical carcinoma(SCC) (0.87%). By single application of a screening program,TCT highest sensitivity (83.3%). Application of any two programs combined screening,TCT+HPV highest sensitivity (94.4%),but the lowest specificity (29.6%) ;TCT or HPV testing combined with hTERC gene, can make both higher sensitivity and specificity. With the increased CIN level of pathology, the detection rate of the screening program gradually increased (P<0.05). Conclusions:In the cervical cancer screening, combined screening is the best way than the single screening, HPV+hTERC joint screening of genetic testing best, but the highest cost, the TCT+HPV screening test regarded as a joint cost-effective screening program,can be carried as a basic screening. Screening should be based on economic conditions and cost options for the screening program.关键词
宫颈肿瘤/早期诊断/组织细胞学制备技术/染色体/端粒,末端转移酶/DNA探针,HPV/原位杂交,荧光/hTERC基因Key words
Uterine cervical neoplasms/Early diagnosis/Histocytological preparation techniques/Chromosomes/ Telomerase / DNA probes, HPV/ In situ hybridization, fluorescence/ Human telomerase RNA component
