高校化学工程学报2011，Vol.25Issue(6)：991-996,6.
人羊膜间充质干细胞在微载体动态培养体系中的扩增和成骨诱导分化
Expansion and Osteogenic Differentiation of Human Amnion Mesenchymal Stem Cell in a Microcarrier-Based Dynamic Culture System
摘要
Abstract
Human amnion mesenchymal stem cells (hAMSCs) are an attractive cell source for bone tissue engineering. However, their utility is restrained by low expansion rate and poor osteogenic differentiation under static culture conditions. In the present study, hAMSCs were seeded and cultured in 12-wells plates as static culture system or Cytodex3 microcarrier-based dynamic culture system. Both proliferation and osteogenic differentiation of hAMSCs in spinner flasks were investigated and compared with those in static plates. Total cell number, alkaline phosphatase activity, and calcium deposition were determined. The effects of fluid flow stimulation and collagen coating on osteogenic differentiation of hAMSCs were further analyzed in static plates. The results show that higher cell density and stronger osteogenic differentiation in microcarrier-based dynamic culture were achieved than those in static plates. It was further found that fluid flow stimulation and collagen coating could promote osteogenic differentiation of hAMSCs, and there exists additive effects when fluid flow stimulation and collagen coating were combined. Therefore, Cytodex 3 microcarrier-based dynamic culture system represents a promising strategy for expansion and osteogenic differentiation of hAMSCs in an integrated manner.关键词
人羊膜间充质干细胞/体外扩增:成骨分化/流体力学刺激/胶原基质Key words
human amnion mesenchymal stem cell/ external expansion/ osteogenic differentiation/ fluid flow stimulation/ collagen coating分类
生物科学引用本文复制引用
周倩,周燕,叶朝阳,谭文松..人羊膜间充质干细胞在微载体动态培养体系中的扩增和成骨诱导分化[J].高校化学工程学报,2011,25(6):991-996,6.基金项目
国家自然科学基金(20776044) （20776044）
上海市自然科学基金(10ZR1407100) （10ZR1407100）
国家重点实验室专项经费资助(2060204). （2060204）
