高校化学工程学报2011，Vol.25Issue(6)：1010-1015,6.DOI:33-1141/TQ.20111104.1426.004
叔戊醇体系中酶法合成L-抗坏血酸脂肪酸酯的研究
Study of Enzymatic Synthesis of L-ascorbyl Fatty Acid Esters in Tert-amyl Alcohol
张洪勇 1钱俊青1
作者信息
- 1. 浙江工业大学药学院,浙江杭州310014
- 折叠
摘要
Abstract
The synthesis of L-ascorbyl fatty acid esters through transesterification of ascorbic acid and palm oil in tert-amy\ alcohol catalyzed by immobilized lipase was studied. A series of solvents used for the reaction were investigated, among them the tert-amyl alcohol was found to be the most suitable reaction media for the enzymatic synthesis of L-ascorbyl fatty acid esters. The addition of the molecular sieves was found to be helpful for reaction system to keep the water activity and to make the equilibrium go to the product. The factors affecting catalytic leaction, such as the substrate concentration, amount of solvent, substrate molar ratio, temperature, water activity and the addition of molecular sieve, were investigated. The results show that, when 0.352 g ascorbic acid and 4.535 g palm oil (the molar ratio of ascorbic acid to fatty acid is 1:8) are catalyzed by 14.2% Novo435 (weight % of ascorbic acid) in 20 mL terf-amyl alcohol with 50 g·L-1 molecular sieve added and rotation speed of 200 r·min-1, the conversion of ascorbic acid can reach 65% and the concentration of L-ascorbyl fatty acid esters is 22 g·L-1 after 24 h reaction at 55℃.关键词
棕榈油/L-抗坏血酸/脂肪酶/酯交换反应/L-抗坏血酸脂肪酸酯/转化率Key words
palm oil/ L-ascorbic acid/ lipase/ transesterification/ L-ascorbyl fatty acid esters/ conversion分类
化学化工引用本文复制引用
张洪勇,钱俊青..叔戊醇体系中酶法合成L-抗坏血酸脂肪酸酯的研究[J].高校化学工程学报,2011,25(6):1010-1015,6.
