广西科学2011，Vol.18Issue(4)：355-358,4.
傅里叶红外光谱法测定罗汉果中罗汉果总甙含量
Determination of Mogrosides Contents in Siraitia grosvenorii with FTIR
摘要
Abstract
Fourier transform infrared spectroscopy (FT-IR) was utilized as a new approach to investigate the mogrosides contents of Siraitia grosvenorii. A serial intensity value of 1059 cm"1 peak,which was intended to be the characteristic peak of mogrosides within its IR fingerprint spectra,was obtained from the IR spectra of different contents of mogrosides standards and used to draw standard curve as a function of mogrosides contents,in which the curve was fitted with least square fitting (LSF) for establishing linear regression equation. The concentration of mogrosides extracted from fructus momordicae sample with methanol combining ultrasonic method was determined by using the regression equation. The relative standard deviation (RSD ) was calculated from 6 replicate determinations, of which the precision, repeatability and stability were 5. 41%, 5. 47% and 5. 47%, respectively. The mogrosides contents measured from different Siraitia grosvenorii samples by FT-IR were not significant differences compared with those determined by UV spectrophotometryC P > 0. 05) , suggesting that FT-IR is a feasible method for quantificational detection of mogrosides content in Siraitia grosvenorii.关键词
罗汉果/总甙/红外光谱/定量分析Key words
Siraitia grosvenorii/ mogrosides/ infrared spectra/ quantitative analysis分类
化学化工引用本文复制引用
郑娟梅,李武钢,毛晓丽,王一兵,叶月华,黄庶识..傅里叶红外光谱法测定罗汉果中罗汉果总甙含量[J].广西科学,2011,18(4):355-358,4.基金项目
广西科学研究与技术开发计划项目(桂科攻0992003A-20)资助. （桂科攻0992003A-20）
