广西科学2011，Vol.18Issue(4)：376-379,4.
拟穴青蟹群体遗传多样性的ISSR分析
Genetic Diversity of Geographic Populations of Scylla Paramamosain Based on ISSR Markers
蔡小辉 1彭银辉 1宋忠魁 1阎冰 1杨家林1
作者信息
- 1. 广西海洋研究所广西海洋生物技术重点实验室,广西北海536000
- 折叠
摘要
Abstract
The molecular marker technique of inter-simple sequence repeat (ISSR)was used to analyze the genetic diversity in three geographic populations of Scylla paramamoain , which were taken from Zhangzhou in Fujian,Fangchenggang and Beihai in Guangxi. A total of 63 loci from 60 individuals were amplified by polymerase-chain-reaction (PGR) with 10 primers. The Number of polymorphic loci were 56. The Nei's gene diversity of Fujian , Beihai and Fangcheng-gang populations was 0. 2670,0. 1819 and 0. 2257,repectively. The/Shannon's information index was 0. 3945,0. 2708 and 0. 3371,repectively,showing that the abundance of genetic diversity in the three populations arranged in a decent order was FJ>FOBH. The Nei's gene diversity and Shannon's information index of Scylla paramamosain was 0. 2248 and 0. 3341 at species, 0.3186 and 0.4337 at population level, respectively, showing that the genetic diversity of S. Paramamosain was relatively high. An analysis of the molecular variance (AMOVA) demonstrated that the among-population component accounted for 32.34% of the total variation, while the within-population component accounted for 67. 66%. The within-population genetic variation was apparently larger than the among-population. The genetic differentiation coefi-cient ( Gst) among the three populations was 0. 1542. There was genetic differentiation between Fujian and the other two populations.关键词
拟穴青蟹/遗传多样性/ISSRKey words
Scylla paramamosain/genetic diversity/ISSR分类
生物科学引用本文复制引用
蔡小辉,彭银辉,宋忠魁,阎冰,杨家林..拟穴青蟹群体遗传多样性的ISSR分析[J].广西科学,2011,18(4):376-379,4.
