广西科学2011，Vol.18Issue(4)：385-391,7.
甘蔗糖蜜酒精高产酵母菌株MF1001的高浓发酵生产试验
High Gravity Alcoholic Fermentation from Sugarcane Molasses by a High-yield Saccharomyces cerevisiae Strain MF1001 in Industrial Scale
摘要
Abstract
A high-yield Saccharomyces cerevisiae strain MF1001 was used for high gravity alcohol fermentation of sugarcane molasses in industrial scale. The fermentation system was composed of 9 fermentors (No. L~No. 9),of which the No. 1 was used for cultivating the strain with low gravity of molasses medium, the No. 2 was used for initiating the fermentation by mixing the low- and high-gravity molasses medium,and the No. 3~No. 9 fermentors were used for continuing the fermentation. The fermentation temperature,controlled by water cycle pipe system equipped within No. 1~4 fermentors, was 30~32°C(No. 1 ) ,34~36℃ (No. 2 and No. 3),30~32°C(No. 4.),33~35℃(No. 5 and No. 6) ,34~36°C (No. 7~~No. 9). The medium flowwas controlled to 64m /h by quantity pumps. The pH value was adjusted at 3. 6~3. 8 for low gravity medium and at 3. 8~4. 0 for high gravity medium respectively. The graity was controlled about 20°Bx for low graity medium and 40~47° Bx for high graity medium. The results from 15-day continuing production showed that,after 49. 5h fermentation,the maximum average alcoholic concentration in broth was 14. 1% (V/V) for shift production and 13. 8% (V/V) for day pro-duction,respectively. Based on fermentable sugar content in medium,the fermentation efficiency of whole production period was calculated,and the average was 97. 72% with the maximum reached to 98. 89% of theoretic value. The waster water effluent for production of 1 ton alcohol (95%, m/V) was decreased to 7. 57 tons at lowest and 8. 17 tons at average. The fermentation level reflected from production parameters was significant better than that of current production in China.关键词
酿酒酵母/菌株MF1001/甘蔗糖蜜/酒精/发酵生产Key words
Saccharomyces cerevisiae/ strains MF1001/ sugarcane molasses/ alcoholic/ fermentation/ production scale分类
生物科学引用本文复制引用
陈东,陆琦,张穗生,陈英,黄日波..甘蔗糖蜜酒精高产酵母菌株MF1001的高浓发酵生产试验[J].广西科学,2011,18(4):385-391,7.基金项目
科技部科技人员服务企业行动项目(2009GJE10002),广西科学研究与技术开发计划项目(桂科合10100019-21、1140010-15,桂科攻1099071,桂科转10123005-17),广西科学院科技攻关项目(桂科院研0708),广西科学院基本科研业务费项目(10YJ25SW15)资助. （2009GJE10002）
