广西林业科学2011，Vol.40Issue(4)：288-291,4.
红锥ISSR—PCR扩增体系的优化
The Optimization of ISSR-PCR Reaction System for Castanopsis hystrix
摘要
Abstract
The DNA and ISSR-PCR amplification of Guangxi Catamopsis hystrix was extracted and optimized respectively. The factors which affected the ISSR amplification such as annealing temperature, template DNA dosage, Mg^2＋ concentration, dNTPs concentration and unit of Taq DNA polymerase were selected and optimized. The results showed that the reaction system being suitable for ISSR - PCR of Castanopsis hystrix was as follows ： 1 × Taq buffer （10 raM/L Tris-HCl , pH 9.0, 50 mM/L KC1 and 0.1% Triton X-100） , 1.25 U Taq DNA polymerase , 0.2 mM 4 8 dNTP, 0.4μM primers, 2.0 mM/L MgCl2 and 50 ng template DNA in total 25 μL reaction volume.关键词
红锥/ISSR/优化/遗传多样性Key words
Castanopsis hystrix/ISSR/optimization/genetic diversity分类
农业科技引用本文复制引用
刘海龙,董民利,杨开太,蒋华,覃子海..红锥ISSR—PCR扩增体系的优化[J].广西林业科学,2011,40(4):288-291,4.基金项目
广西林科院基本科研业务费项目(林科200901) （林科200901）
