南方农业学报2011，Vol.42Issue(10)：1181-1184,4.
酿酒葡萄桂葡一号花粉原生质体分离条件初探
Isolation of protoplasts from pollens of wine grape variety Guipu 1
摘要
Abstract
[Objective]The aim of this experiment was to find out suitable conditions for isolation of mature protoplasts from wine grape pollens in order to facilitate protoplast hybridization for improving wine grape cultivars. [Method]The mature protoplasts of pollens taken from grape wine variety Cuipu 1 were isolated using cold pretreatment and enzyme decomposition methods. The effects of different duration of low temperatures, different inflorescence positions and mannitol concentrations on its isolation were determined. [Result]The results indicated that the rate of protoplast isolation increased with elongation of low temperature (4℃) duration, the highest rate was recorded as 19.40% after treating pollens 7 days with 0.6 M mannitol, followed by 18.94% after treating pollens for 5 days. The rate of protoplast isolation from anthers of top inflorescence was the highest (19.11%), and that of protoplasts from anther of middle and lower inflorescence was lower. Compared to 1.2 M mannitol, the rate of protoplast isolation was higher in 0.6 M mannitol. [Conclusion]The low temperature duration, pollen maturity and mannitol concentration have significant effects on isolation of protoplast from pollen. The highest protoplast may be obtained from pollens of middle and upper part of anther and treating them for 5-7 days under 0-4℃ low temperature with 0.6 M mannitol.关键词
酿酒葡萄/桂萄一号/花粉/原生质体/分离条件Key words
Guipu 1/ pollen/ protoplast/ isolation/ wine grape分类
农业科技引用本文复制引用
廖芬,唐文忠,黄茂康,周主贵..酿酒葡萄桂葡一号花粉原生质体分离条件初探[J].南方农业学报,2011,42(10):1181-1184,4.基金项目
广西自然科学基金项目(桂科自0640023) （桂科自0640023）
